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中华腔镜泌尿外科杂志(电子版) ›› 2023, Vol. 17 ›› Issue (05) : 516 -522. doi: 10.3877/cma.j.issn.1674-3253.2023.05.018

所属专题: 总编推荐

实验研究

不同比例聚乳酸/丝素蛋白复合支架在兔尿道缺损修复中的疗效
钟文文, 李科, 刘碧好, 蔡炳, 脱颖, 叶雷, 马波, 瞿虎, 汪中扬, 王德娟(), 邱剑光   
  1. 510655 广州,中山大学附属第六医院泌尿外科
    510630 广州,中山大学附属第三医院泌尿外科
    510062 广州,中山大学附属第一医院生殖医学科
    510062 广州,中山大学附属第一医院病理科
  • 收稿日期:2022-06-23 出版日期:2023-10-01
  • 通信作者: 王德娟
  • 基金资助:
    广东省自然科学基金面上项目(2019A1515010386)

Comparison of the efficacy of composite scaffolds with different proportions of polylactic acid and silk fibroin in urethral repair in rabbits

Wenwen Zhong, Ke Li, Bihao Liu, Bing Cai, Yin Tuo, Lei Ye, Bo Ma, Hu Qu, Zhongyang Wang, Dejuan Wang(), Jianguang Qiu   

  1. Department of Urology, the Sixth Affiliated Hospital of Sun Yat-sen University, Guangzhou 510655, China
    Department of Urology, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China
    Department of Reproductive Medicine, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510062, China
    Department of Pathology, the First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510062, China
  • Received:2022-06-23 Published:2023-10-01
  • Corresponding author: Dejuan Wang
引用本文:

钟文文, 李科, 刘碧好, 蔡炳, 脱颖, 叶雷, 马波, 瞿虎, 汪中扬, 王德娟, 邱剑光. 不同比例聚乳酸/丝素蛋白复合支架在兔尿道缺损修复中的疗效[J/OL]. 中华腔镜泌尿外科杂志(电子版), 2023, 17(05): 516-522.

Wenwen Zhong, Ke Li, Bihao Liu, Bing Cai, Yin Tuo, Lei Ye, Bo Ma, Hu Qu, Zhongyang Wang, Dejuan Wang, Jianguang Qiu. Comparison of the efficacy of composite scaffolds with different proportions of polylactic acid and silk fibroin in urethral repair in rabbits[J/OL]. Chinese Journal of Endourology(Electronic Edition), 2023, 17(05): 516-522.

目的

对比不同比例的3D打印聚乳酸及丝素蛋白复合支架在兔尿道缺损修复中的疗效。

方法

构建聚乳酸(PLA)/丝素蛋白(SF)比例分别为25∶75、50∶50、75∶25的复合支架材料,与诱导多能干细胞共培养。24只人工尿道下裂模型的雄性新西兰兔随机分为3组,分别采用不同比例的复合支架行尿道成形术。在尿道成形术后6周,收集尿道移植物并进行大体和组织学分析,统计尿道狭窄的发生率。

结果

体外实验中随着SF在复合材料中的比例增高,细胞黏附、增殖的数量增加。术后6周,PLA/SF=25∶75比例组尿道狭窄发生率低于其他两组(P<0.05)。

结论

25∶75比例的聚乳酸/丝素蛋白复合材料更加有助于尿道缺损的修复。

Objective

To compare the effect of 3D printing composite scaffolds with different proportions of polylactic acid (PLA) and silk fibroin (SF) in urethral repair in rabbits.

Methods

The composite scaffolds were built with different ratios of PLA and SF (25∶75, 50∶50, 75∶25), and the Urethral epithelial cells that from stem cells were co-cultured on the composite scaffolds. 24 male rabbits with artificial hypospadias were randomly divided into 3 groups, and treated by urethroplasty with different composite scaffolds. Urethral grafts were collected for histological analysis 6 weeks after urethroplasty, and the incidence of urethral stricture was calculated.

Results

As the ratios of SF in the composite increased, the number of cells that adhered to the composite and propagated on the composite increased in vitro. Six weeks after urethroplasty, the incidence of urethral stricture in the group that proportion of PLA and SF 25∶75 was lower than that in the other two groups (P<0.05).

Conclusion

The composite scaffolds, which proportion of PLA and SF was 25∶75, were more helpful for urethral defect repair.

图1 诱导多能干细胞(iPSC)的多能性鉴定注:经免疫组化鉴定,诱导后的细胞可表达多能基因标志OCT4、TRA-1-60、SSEA4(bar=100 μm),表明已成功诱导多能干细胞
图2 iPSC诱导分化为尿路上皮细胞注:在诱导分化的21 d分化细胞大部分出现上皮细胞鹅卵石结构样细胞(a),免疫组化显示该分化细胞存在UP1a阳性(b)、UP1b阳性(c)、UP2阳性(d)细胞(bar=100 μm),经流式细胞仪检测发现UP1a阳性比例为75.8%(e)
图3 干细胞与聚乳酸/丝素蛋白复合材料共培养后1周,碱性磷酸酶染色及HE染色(1∶100)观察图片
图4 术后6周,聚乳酸/丝素蛋白复合材料的皮下种植物及尿道修补组织的HE染色(1∶100)观察图片
表1 术后6周出现尿道狭窄或尿道闭锁的新西兰兔数量
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