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中华腔镜泌尿外科杂志(电子版) ›› 2017, Vol. 11 ›› Issue (01) : 66 -69. doi: 10.3877/cma.j.issn.1674-3253.2017.01.016

所属专题: 文献

实验研究

WAVE1和高迁移率族蛋白-1在兔尿源性脓毒血症肺组织内的表达关系
张学齐1, 袁也晴1, 魏育英1, 郭吉楠1, 黄东龙1, 田素萍1, 杨江根1, 张轶庠,1   
  1. 1. 518020 广东,深圳市人民医院泌尿外科、暨南大学附属第二临床医学院
  • 收稿日期:2015-02-13 出版日期:2017-02-01
  • 通信作者: 张轶庠
  • 基金资助:
    广东省医学科研基金(A2014634); 深圳市卫生计生系统科研项目(201401002)

The relationship between WAVE1 and high mobility group box 1 in lung tissues in septic rabbits

Xueqi Zhang1, Yeqing Yuan1, Yuying Wei1, Jinan Guo1, Donglong Huang1, Suping Tian1, Jianggen Yang1, Yixiang Zhang,1   

  1. 1. Department of Urology, Shenzhen People's Hospital, the Second Affiliated Hospital, Jinan University, Shenzhen 518020, China
  • Received:2015-02-13 Published:2017-02-01
  • Corresponding author: Yixiang Zhang
  • About author:
    Correspondence author: Zhang Yixiang,
引用本文:

张学齐, 袁也晴, 魏育英, 郭吉楠, 黄东龙, 田素萍, 杨江根, 张轶庠. WAVE1和高迁移率族蛋白-1在兔尿源性脓毒血症肺组织内的表达关系[J/OL]. 中华腔镜泌尿外科杂志(电子版), 2017, 11(01): 66-69.

Xueqi Zhang, Yeqing Yuan, Yuying Wei, Jinan Guo, Donglong Huang, Suping Tian, Jianggen Yang, Yixiang Zhang. The relationship between WAVE1 and high mobility group box 1 in lung tissues in septic rabbits[J/OL]. Chinese Journal of Endourology(Electronic Edition), 2017, 11(01): 66-69.

目的

探讨WASP家族Verprolin同源蛋白1(WAVE1)和高迁移率族蛋白-1(HMGB1)在兔尿源性脓毒血症肺组织中表达上调中的关系。

方法

30只健康雄性家兔随机分为假手术组(Sham组)和尿源性脓毒血症组(US组)。采用肾盂内高压法制备家兔尿源性脓毒血症模型。免疫组织化学染色SABC法分别于术后12 h、24 h、36 h检测家兔肺组织WAVE1蛋白表达水平及肺组织中HMGB1含量及HMGB1 mRNA表达。

结果

与Sham组比较,US组肺组织HMGBl含量、HMGBl mRNA表达活性升高(P<0.05);免疫组织化学结果显示,US组肺组织中WAVE1 80.00%(12/15))呈强阳性表达,在Sham组肺组织中,WAVE1 86.67%(13/15))呈低表达或不表达,差异具有统计学意义(P<0.05)。

结论

尿源性脓毒血症急性肺损伤中WAVE1与HMGB1可能具有相关性。

Objective

To explore the relationship between WASP-family verprolin-homologous protein 1 and high mobility group box 1 in lung tissues in septic rabbits.

Methods

Thirty healthy male rabbits were randomly divided into 2 groups: sham operation group (Sham group), urosepsis group (US group). The model of urpsepsis was induced by acute pelvic obstruction in high pressure. SABC immunohistochemistry was done respectively to determinate WAVE1 protein expression levels, HMGBl content and HMGBl mRNA expression in lung tissues at 12 h, 24 h and 36 h after operation.

Results

Compared with Sham group, HMGBl content and HMGBl mRNA expression were significantly increased in US group (P<0.05). WAVE1 was strongly positively expressed in 80% of US group, while it was weakly positive or negative expressed in 86.67%of Sham group. The differences between the two groups were statistically significant (P<0.05).

Conclusions

WAVE1 potentially involved in the up-regulation of HMGBl expression in lung tissues in septic rabbits.

表1 两组术后12 h、24 h、36 h每个时间点处死的五只家兔的HMGBl含量、HMGBl mRNA表达情况(±s)
表2 Sham组与US组家兔肺组织WAVE1表达情况(例)
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