切换至 "中华医学电子期刊资源库"

中华腔镜泌尿外科杂志(电子版) ›› 2017, Vol. 11 ›› Issue (01) : 66 -69. doi: 10.3877/cma.j.issn.1674-3253.2017.01.016

所属专题: 文献

实验研究

WAVE1和高迁移率族蛋白-1在兔尿源性脓毒血症肺组织内的表达关系
张学齐1, 袁也晴1, 魏育英1, 郭吉楠1, 黄东龙1, 田素萍1, 杨江根1, 张轶庠1,()   
  1. 1. 518020 广东,深圳市人民医院泌尿外科、暨南大学附属第二临床医学院
  • 收稿日期:2015-02-13 出版日期:2017-02-01
  • 通信作者: 张轶庠
  • 基金资助:
    广东省医学科研基金(A2014634); 深圳市卫生计生系统科研项目(201401002)

The relationship between WAVE1 and high mobility group box 1 in lung tissues in septic rabbits

Xueqi Zhang1, Yeqing Yuan1, Yuying Wei1, Jinan Guo1, Donglong Huang1, Suping Tian1, Jianggen Yang1, Yixiang Zhang1,()   

  1. 1. Department of Urology, Shenzhen People's Hospital, the Second Affiliated Hospital, Jinan University, Shenzhen 518020, China
  • Received:2015-02-13 Published:2017-02-01
  • Corresponding author: Yixiang Zhang
  • About author:
    Correspondence author: Zhang Yixiang,
引用本文:

张学齐, 袁也晴, 魏育英, 郭吉楠, 黄东龙, 田素萍, 杨江根, 张轶庠. WAVE1和高迁移率族蛋白-1在兔尿源性脓毒血症肺组织内的表达关系[J]. 中华腔镜泌尿外科杂志(电子版), 2017, 11(01): 66-69.

Xueqi Zhang, Yeqing Yuan, Yuying Wei, Jinan Guo, Donglong Huang, Suping Tian, Jianggen Yang, Yixiang Zhang. The relationship between WAVE1 and high mobility group box 1 in lung tissues in septic rabbits[J]. Chinese Journal of Endourology(Electronic Edition), 2017, 11(01): 66-69.

目的

探讨WASP家族Verprolin同源蛋白1(WAVE1)和高迁移率族蛋白-1(HMGB1)在兔尿源性脓毒血症肺组织中表达上调中的关系。

方法

30只健康雄性家兔随机分为假手术组(Sham组)和尿源性脓毒血症组(US组)。采用肾盂内高压法制备家兔尿源性脓毒血症模型。免疫组织化学染色SABC法分别于术后12 h、24 h、36 h检测家兔肺组织WAVE1蛋白表达水平及肺组织中HMGB1含量及HMGB1 mRNA表达。

结果

与Sham组比较,US组肺组织HMGBl含量、HMGBl mRNA表达活性升高(P<0.05);免疫组织化学结果显示,US组肺组织中WAVE1 80.00%(12/15))呈强阳性表达,在Sham组肺组织中,WAVE1 86.67%(13/15))呈低表达或不表达,差异具有统计学意义(P<0.05)。

结论

尿源性脓毒血症急性肺损伤中WAVE1与HMGB1可能具有相关性。

Objective

To explore the relationship between WASP-family verprolin-homologous protein 1 and high mobility group box 1 in lung tissues in septic rabbits.

Methods

Thirty healthy male rabbits were randomly divided into 2 groups: sham operation group (Sham group), urosepsis group (US group). The model of urpsepsis was induced by acute pelvic obstruction in high pressure. SABC immunohistochemistry was done respectively to determinate WAVE1 protein expression levels, HMGBl content and HMGBl mRNA expression in lung tissues at 12 h, 24 h and 36 h after operation.

Results

Compared with Sham group, HMGBl content and HMGBl mRNA expression were significantly increased in US group (P<0.05). WAVE1 was strongly positively expressed in 80% of US group, while it was weakly positive or negative expressed in 86.67%of Sham group. The differences between the two groups were statistically significant (P<0.05).

Conclusions

WAVE1 potentially involved in the up-regulation of HMGBl expression in lung tissues in septic rabbits.

表1 两组术后12 h、24 h、36 h每个时间点处死的五只家兔的HMGBl含量、HMGBl mRNA表达情况(±s)
表2 Sham组与US组家兔肺组织WAVE1表达情况(例)
[1]
Bjerklund Johansen TE, Cek M, Naber K, et al. Prevalence of hospital-acquired urinary tract infections in urology departments[J]. Eur Urol, 2007, 51(4): 1100-1111; discussion 1112.
[2]
向松涛,王树声,甘澍等.经皮肾镜取石术后尿脓毒症休克的诊治特点分析[J].中华泌尿外科杂志, 2010, 31(8): 520-523.
[3]
李天,李逊,何永忠,等.微创经皮肾镜取石术后并发感染性休克的诊治体会[J].临床泌尿外科杂志, 2012, 27(3): 182-183, 187.
[4]
Iscimen R, Cartin-Ceba R, Yilmaz M, et al. Risk factors for the development of acute lung injury in patients with septic shock: an observational cohort study[J]. Crit Care Med, 2008, 36(5): 1518-1522.
[5]
徐丽,王晓亮,韩流,等.血红素加氧酶-1在脓毒症大鼠炎性反应中的作用[J].中华麻醉学杂志, 2012, 32(4): 494-496.
[6]
Henderson B, Nair SP. Hard Labour: bacterial infection of the skeleton[J]. Trends Microbiol, 2003, 11(12): 570-577.
[7]
Namekata K1, Harada C, Taya C, et al. Dock3 induces axonal outgrowth by stimulating membrane recruitment of the WAVE complex[J]. Proc Natl Acad Sci U S A, 2010, 107(16): 7586-7591.
[8]
许武军,陈仙,罗志刚,等. H_2S对尿源性脓毒血症肾损伤的影响[J].天津医药, 2014, 42(8): 769-773, 850.
[9]
陈继红,熊苗,杨宾烈,等.诱骗受体3在子宫颈上皮内瘤变及子宫颈癌中的表达及临床意义[J].中华妇产科杂志, 2012, 47(2): 150-152.
[10]
Zanetti G, Paparella S, Trinchieri A, et a1.Infections and urolithiasis: current clinical evidence in prophylaxis and antibiotic therapy[J]. Archhal Urol Androl, 2008, 80(1): 5-12.
[11]
Sims GP, Rowe DC, Rietdijk ST, et al. HMGB1 and RAGE in inflammation and cancer[J]. Annu Rev Immunol, 2010, 28: 367-388.
[12]
Yanai H, Ban T, Wang Z, et al. HMGB proteins function as Universal sentinels for nucleic-acid-mediated innate immune responses[J]. Nature, 2009, 462(7269): 99-103.
[13]
Kang R, Tang D, Yu Y, et al. WAVE1 regulates Bcl-2 localization and phosphorylation in leukemia cells[J]. Leukemia, 2010, 24(1): 177-186.
[14]
Dinh H, Scholz GM, Hamilton JA. Regulation of WAVE1 expression in macrophages at multiple levels[J]. J Leukoc Biol, 2008, 84(6): 1483-1491.
[15]
Gardella SA, Rubartelli A. The nuclear protein HMGB1 is secreted by monocytes via a non-classical,vesicle-mediated secretory pathway[J]. EMBO Rep, 2012, 13(10): 995-1001.
[16]
Oh YJ1, Youn JH, Ji Y, et al. HMGB1 is phosphorylated by classical protein kinase C and is secreted by a calcium-dependent mechanism[J]. J Immunol, 2009, 182(9): 5800-5809.
[17]
Tang D, Shi Y, Kang R, et al. Hydrogen peroxide stimulates macrophages and monocytes to actively release HMGB1[J]. J Leukoc Biol, 2007, 81(3): 741-747.
[18]
Welk B, Fuller A, Razvi H. Quercetin prevents LPS-induced high-mobility group box 1 release and proinflammatory function[J]. Am J Respir Cell Mol Biol, 2009, 41(6): 651-660.
[19]
Tang D, Kang R, Xiao W, et al. Nuclear heat shock protein 72 as a negative regulator of oxidative stress (Hydrogen peroxide)-induced HMGB1 cytoplasmic translocation and release[J]. J Immunol, 2007, 178(11): 7376-7384.
[1] 梁哲浩, 方明笋, 胡弘毅, 陶涛, 徐孝平, 孙华琴. 基于生物信息学分析筛选脓毒症诱导急性肺损伤的关键基因[J]. 中华危重症医学杂志(电子版), 2022, 15(05): 360-366.
[2] 赵希伟, 周佳伟, 刘凯, 侯林义, 张文凯. 连接蛋白43通过蛋白激酶A介导丝氨酸373调控脓毒症急性肺损伤肺泡Ⅱ型上皮细胞屏障功能的研究[J]. 中华危重症医学杂志(电子版), 2021, 14(05): 355-361.
[3] 沈纵, 魏晨如, 朱邦晖, 包郁露, 伍国胜, 孙瑜. 间充质干细胞治疗吸入性损伤的动物实验研究进展[J]. 中华损伤与修复杂志(电子版), 2023, 18(02): 180-183.
[4] 蔡维霞, 曹涛, 赵明, 肖丹, 贾艳慧, 王璟, 张月, 王克甲, 韩军涛, 胡大海. Notch信号通路对烧伤大鼠血清诱导的肺血管内皮细胞细胞间黏附分子-1的影响[J]. 中华损伤与修复杂志(电子版), 2022, 17(04): 292-299.
[5] 周强, 赵烨德, 王雨翔, 肖仕初. 烧伤合并烟雾吸入性肺损伤病理机制和治疗研究新进展[J]. 中华损伤与修复杂志(电子版), 2022, 17(02): 171-175.
[6] 伍学成, 李远伟, 袁武雄, 王建松, 石泳中, 卢强, 李卓, 陈佳, 刘哲, 滕伊漓, 高智勇. 炎症介质谱联合降钙素原在尿源性脓毒血症中的诊断价值[J]. 中华腔镜泌尿外科杂志(电子版), 2023, 17(05): 476-480.
[7] 吴小凤, 陈丽茹, 程欣, 冯钰, 邓含, 张玲. 标准化情景演练模式在PCNL术后尿源性脓毒血症患者救治中的应用价值[J]. 中华腔镜泌尿外科杂志(电子版), 2022, 16(03): 214-218.
[8] 吴少辉, 严兵, 何立彬, 杜佳恒, 薛康颐. 经皮肾镜致尿源性脓毒血症的危险因素及最佳干预时机的初步探讨[J]. 中华腔镜泌尿外科杂志(电子版), 2022, 16(03): 210-213,218.
[9] 熊欢庆, 李玉娟, 陈键, 刘刚, 李志超, 金发光. 丹参酮IIA及苦参碱组方对脂多糖致小鼠急性肺损伤的协同保护作用[J]. 中华肺部疾病杂志(电子版), 2023, 16(04): 455-459.
[10] 李埝, 赵建军, 张建勇, 赵睿桢. hAMSCs调控MAPK信号通路对急性肺损伤AQP1的影响[J]. 中华肺部疾病杂志(电子版), 2023, 16(02): 156-163.
[11] 朱冠能, 汪洋, 宋海苗, 汪骏东. 血清铁蛋白及C反应蛋白水平对胸部创伤后急性肺损伤的预测意义[J]. 中华肺部疾病杂志(电子版), 2022, 15(03): 379-381.
[12] 许发琼, 贺斌峰, 黄朝旺, 胡明冬. 非编码RNA调控巨噬细胞炎症反应在ALI/ARDS中的研究进展[J]. 中华肺部疾病杂志(电子版), 2021, 14(05): 677-680.
[13] 陈梦婷, 孟潇潇, 王瑞兰. 急性肺损伤时肺部微环境介导的细胞代谢变化的研究进展[J]. 中华重症医学电子杂志, 2022, 08(01): 80-84.
[14] 苟扬, 姜庆. 肾结石行经皮肾镜取石术的临床应用及术后尿源性脓毒血症发生的危险因素分析[J]. 中华临床医师杂志(电子版), 2022, 16(02): 183-186.
[15] 胡俊晟, 黄荣, 黄毅, 曾光, 金永志, 李梦帆. 丹参多酚酸盐通过Nrf2/HO-1信号通路对脂多糖诱导的小鼠急性肺损伤的保护作用[J]. 中华临床医师杂志(电子版), 2021, 15(12): 1024-1030.
阅读次数
全文


摘要