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中华腔镜泌尿外科杂志(电子版) ›› 2022, Vol. 16 ›› Issue (04) : 351 -355. doi: 10.3877/cma.j.issn.1674-3253.2022.04.015

实验研究

PGE2通过促进EP4受体表达调控脂多糖诱导的膀胱上皮细胞炎症反应
周益红1, 吕夷松2, 程文杰1, 郑小青1, 黄书畅1, 延敏博1, 戴英波1, 郑浩1,()   
  1. 1. 519000 珠海,中山大学附属第五医院泌尿外科;519000 珠海,广东省生物医学影像重点实验室
    2. 350000 福州,福能集团总医院泌尿外科
  • 收稿日期:2021-04-15 出版日期:2022-08-01
  • 通信作者: 郑浩
  • 基金资助:
    广东省珠海市科技计划项目(20161027E030052)

PGE2 regulates lipopolysaccharide-induced inflammatory response in bladder epithelial cells by promoting EP4 receptor expression

Yihong Zhou1, Yisong Lyu2, Wenjie Cheng1, Xiaoqing Zheng1, Shuchang Huang1, Minbo Yan1, Yingbo Dai1, Hao Zheng1,()   

  1. 1. Department of Urology, the Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai 519000, China; Guangdong Provincial Key Laboratory of Biomedical Imaging, the Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai 519000, China
    2. Department of Urology, Funeng General Hospital, Fuzhou 350000, China
  • Received:2021-04-15 Published:2022-08-01
  • Corresponding author: Hao Zheng
引用本文:

周益红, 吕夷松, 程文杰, 郑小青, 黄书畅, 延敏博, 戴英波, 郑浩. PGE2通过促进EP4受体表达调控脂多糖诱导的膀胱上皮细胞炎症反应[J/OL]. 中华腔镜泌尿外科杂志(电子版), 2022, 16(04): 351-355.

Yihong Zhou, Yisong Lyu, Wenjie Cheng, Xiaoqing Zheng, Shuchang Huang, Minbo Yan, Yingbo Dai, Hao Zheng. PGE2 regulates lipopolysaccharide-induced inflammatory response in bladder epithelial cells by promoting EP4 receptor expression[J/OL]. Chinese Journal of Endourology(Electronic Edition), 2022, 16(04): 351-355.

目的

探讨PGE2对脂多糖诱导的膀胱上皮细胞炎症反应的影响及相关机制。

方法

利用脂多糖诱导人正常膀胱上皮细胞(SV-HUC-1)建立膀胱炎细胞模型,采用实时荧光定量PCR检测PGE2作用后膀胱上皮细胞中EP4受体的表达水平变化。利用EP4受体激动剂和拮抗剂作用于膀胱上皮细胞,MTT方法检测其对细胞增殖的影响。最后分别利用PGE2及EP4受体激动剂、拮抗剂作用于膀胱上皮细胞,ELISA方法检测细胞培养液中IL-10、IL-6、IL-1β和TNF-α的变化。

结果

PGE2促进膀胱上皮细胞中EP4受体的表达,并呈时间依赖。EP4受体激动剂(CAY10598)促进膀胱上皮细胞的细胞增殖,而EP4受体拮抗剂(AH23848)抑制细胞增殖(P<0.05)。ELISA法检测发现,PGE2和CAY10598能促进膀胱上皮细胞培养液中IL-10、IL-6、IL-1β和TNF-α等炎症因子的表达,而AH23848则起到抑制作用(P<0.05)。

结论

PGE2通过上调EP4受体表达促进脂多糖诱导的膀胱上皮细胞炎症反应,PGE2/EP4受体有望是膀胱炎治疗的潜在靶点。

Objective

To explore the effect and related mechanisms of PGE2 on lipopolysaccharide-induced inflammation of bladder epithelial cells.

Method

Normal human bladder epithelial cell (SV-HUC-1) were induced by lipopolysaccharide to establish a cystitis cell model, and real-time fluorescent quantitative PCR was used to detect the expression of EP4 receptor after the use of PGE2. With the treatment of EP4 receptor agonist and antagonist, MTT method was used to detect the effect on cell proliferation. Finally, the expression of IL-10, IL-6, IL-1β and TNF-α in cell culture medium were detected by ELISA method after the treatment of PGE2, EP4 receptor agonist and antagonist.

Results

PGE2 promoted the expression of EP4 receptor in bladder epithelial cell. EP4 receptor agonist (CAY10598) promoted cell proliferation, while EP4 receptor antagonist (AH23848) inhibited cell proliferation (P<0.05). Furthermore, PGE2 and CAY10598 promoted the expression of IL-10, IL-6, IL-1β and TNF-α, while AH23848 had an inhibitory effect (P<0.05).

Conclusion

PGE2 promotes lipopolysaccharide-induced inflammatory response in bladder epithelial cells by up-regulating the expression of EP4 receptor. And PGE2/EP4 receptor is expected to be a potential target for the treatment of cystitis.

图5 PGE2、EP4受体激动剂和拮抗剂对SV-HUC-1细胞炎症因子表达的影响
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