尿液细胞外囊泡circRNA分类器对高级别前列腺癌诊断价值的初步研究

doi:10.3877/cma.j.issn.1674-3253.2023.04.006

中华腔镜泌尿外科杂志(电子版) ›› 2023, Vol. 17 ›› Issue (04) : 339 -342. doi: 10.3877/cma.j.issn.1674-3253.2023.04.006

临床研究

尿液细胞外囊泡circRNA分类器对高级别前列腺癌诊断价值的初步研究

王邦郁, 陈晓鹏, 唐国军, 王佳妮^†(

)

510630　广州，中山大学附属第三医院甲乳外科
中山大学附属第三医院泌尿外科

收稿日期:2023-05-01 出版日期:2023-08-01
通信作者: 王佳妮
基金资助:
国家自然科学基金(81502268)

A preliminary study of the diagnostic value of urine extracellular vesicle circRNA classifier for prostate cancer

Bangyu Wang, Xiaopeng Chen, Guojun Tang, Jiani Wang^†()

Department of Thyroid and breast, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China
Department of Urology, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China

Received:2023-05-01 Published:2023-08-01
Corresponding author: Jiani Wang

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目的

检验尿液细胞外囊泡环状RNA（circRNA）分类器（Ccirc）在PSA>2 ng/ml患者中的应用效能，进而评估其在高级别前列腺癌诊断中的潜在应用价值。

方法

收集23例高级别前列腺癌和17例低级别前列腺癌/前列腺增生患者尿液，运用第三代微滴数字PCR技术来测定他们尿液中的细胞外囊泡circRNA表达水平。以前列腺活体组织病理结果为金标准，利用受试者工作特征（ROC）曲线评估尿液细胞外囊泡circRNA分类器（Ccirc）和PSA诊断高级别前列腺癌的效果。

结果

高级别前列腺癌组和低级别前列腺癌/前列腺增生组Ccirc表达量分别为（7.78±1.04）copies/ml和（6.63±1.18）copies/ml，高级别前列腺癌组Ccirc表达量高于低级别前列腺癌/前列腺增生组（P=0.003）；尿液细胞外囊泡circRNA分类器和PSA诊断高级别前列腺癌的ROC曲线下面积分别为0.757（95%CI 0.596~0.878）、0.586（95%CI 0.519~0.639）。

结论

尿液细胞外囊泡circRNA分类器（Ccirc）对诊断PSA>2 ng/ml的高级别前列腺癌患者具有良好的效能。

关键词: 前列腺癌, 环状RNA, 细胞外囊泡, 诊断

Objective

To examine the efficacy of the urine extracellular vesicle circRNA classifier (Ccirc) in patients with PSA>2 ng/ml to evaluate its potential application in prostate cancer diagnosis.

Methods

Twenty-three high-grade prostate cancer cases and 17 low-grade prostate cancer/prostatic hyperplasia cases were screened to measure extracellular vesicle circRNA expression in their urine using third-generation droplet digital PCR technology. The pathological results of prostate biopsy (biopsy) were used as judging criteria, and the effect of urine extracellular vesicle circRNA classifier (Ccirc) and PSA in the diagnosis of high-grade prostate cancer was evaluated using the receiver working characteristics (ROC) curve.

Results

The expression of Ccirc in the high-grade prostate cancer group and the low-grade prostate cancer/prostatic hyperplasia group were (7.78±1.04) copies/ml and (6.63±1.18) copies/ml, respectively, and the Ccirc expression in the high-grade prostate cancer group was higher than that in the low-grade prostate cancer/prostatic hyperplasia group (P=0.003). The area under the ROC curve for the diagnosis of high-grade prostate cancer by urine extracellular vesicle circRNA classifier and PSA was (95%CI 0.596-0.878) and 0.586(95%CI 0.419-0.739), respectively.

Conclusion

The urine extracellular vesicle circRNA classifier (Ccirc) has good efficacy in diagnosing high-grade prostate cancer patients with PSA>2 ng/ml.

Key words: Prostate Cancer, Circular RNA, Extracellular Vesicles, Diagnosis

图1 两组患者的Ccirc表达情况

图1 尿液细胞外囊泡circRNA分类器和PSA诊断高级别前列腺癌的ROC曲线

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