切换至 "中华医学电子期刊资源库"

中华腔镜泌尿外科杂志(电子版) ›› 2023, Vol. 17 ›› Issue (04) : 339 -342. doi: 10.3877/cma.j.issn.1674-3253.2023.04.006

临床研究

尿液细胞外囊泡circRNA分类器对高级别前列腺癌诊断价值的初步研究
王邦郁, 陈晓鹏, 唐国军, 王佳妮()   
  1. 510630 广州,中山大学附属第三医院甲乳外科
    中山大学附属第三医院泌尿外科
  • 收稿日期:2023-05-01 出版日期:2023-08-01
  • 通信作者: 王佳妮
  • 基金资助:
    国家自然科学基金(81502268)

A preliminary study of the diagnostic value of urine extracellular vesicle circRNA classifier for prostate cancer

Bangyu Wang, Xiaopeng Chen, Guojun Tang, Jiani Wang()   

  1. Department of Thyroid and breast, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China
    Department of Urology, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China
  • Received:2023-05-01 Published:2023-08-01
  • Corresponding author: Jiani Wang
引用本文:

王邦郁, 陈晓鹏, 唐国军, 王佳妮. 尿液细胞外囊泡circRNA分类器对高级别前列腺癌诊断价值的初步研究[J/OL]. 中华腔镜泌尿外科杂志(电子版), 2023, 17(04): 339-342.

Bangyu Wang, Xiaopeng Chen, Guojun Tang, Jiani Wang. A preliminary study of the diagnostic value of urine extracellular vesicle circRNA classifier for prostate cancer[J/OL]. Chinese Journal of Endourology(Electronic Edition), 2023, 17(04): 339-342.

目的

检验尿液细胞外囊泡环状RNA(circRNA)分类器(Ccirc)在PSA>2 ng/ml患者中的应用效能,进而评估其在高级别前列腺癌诊断中的潜在应用价值。

方法

收集23例高级别前列腺癌和17例低级别前列腺癌/前列腺增生患者尿液,运用第三代微滴数字PCR技术来测定他们尿液中的细胞外囊泡circRNA表达水平。以前列腺活体组织病理结果为金标准,利用受试者工作特征(ROC)曲线评估尿液细胞外囊泡circRNA分类器(Ccirc)和PSA诊断高级别前列腺癌的效果。

结果

高级别前列腺癌组和低级别前列腺癌/前列腺增生组Ccirc表达量分别为(7.78±1.04)copies/ml和(6.63±1.18)copies/ml,高级别前列腺癌组Ccirc表达量高于低级别前列腺癌/前列腺增生组(P=0.003);尿液细胞外囊泡circRNA分类器和PSA诊断高级别前列腺癌的ROC曲线下面积分别为0.757(95%CI 0.596~0.878)、0.586(95%CI 0.519~0.639)。

结论

尿液细胞外囊泡circRNA分类器(Ccirc)对诊断PSA>2 ng/ml的高级别前列腺癌患者具有良好的效能。

Objective

To examine the efficacy of the urine extracellular vesicle circRNA classifier (Ccirc) in patients with PSA>2 ng/ml to evaluate its potential application in prostate cancer diagnosis.

Methods

Twenty-three high-grade prostate cancer cases and 17 low-grade prostate cancer/prostatic hyperplasia cases were screened to measure extracellular vesicle circRNA expression in their urine using third-generation droplet digital PCR technology. The pathological results of prostate biopsy (biopsy) were used as judging criteria, and the effect of urine extracellular vesicle circRNA classifier (Ccirc) and PSA in the diagnosis of high-grade prostate cancer was evaluated using the receiver working characteristics (ROC) curve.

Results

The expression of Ccirc in the high-grade prostate cancer group and the low-grade prostate cancer/prostatic hyperplasia group were (7.78±1.04) copies/ml and (6.63±1.18) copies/ml, respectively, and the Ccirc expression in the high-grade prostate cancer group was higher than that in the low-grade prostate cancer/prostatic hyperplasia group (P=0.003). The area under the ROC curve for the diagnosis of high-grade prostate cancer by urine extracellular vesicle circRNA classifier and PSA was (95%CI 0.596-0.878) and 0.586(95%CI 0.419-0.739), respectively.

Conclusion

The urine extracellular vesicle circRNA classifier (Ccirc) has good efficacy in diagnosing high-grade prostate cancer patients with PSA>2 ng/ml.

图1 两组患者的Ccirc表达情况
图1 尿液细胞外囊泡circRNA分类器和PSA诊断高级别前列腺癌的ROC曲线
[1]
朱学华, 邵立智, 颜野等. 前列腺癌根治术后生化复发相关MRI影像组学特征的研究[J]. 中国微创外科杂志, 2023, 23(5): 359-366.
[2]
Aranha O, Vaishampayan U. PSA relapse prostate cancer: the importance of tailored therapy[J]. Urol Oncol, 2004, 22(1): 62-69.
[3]
Cocozza F, Grisard E, Martin-Jaular L, et al. SnapShot: Extracellular Vesicles[J]. Cell, 2020, 182(1): 262-262. e1.
[4]
Tóth , Turiák L, Visnovitz T, et al. Formation of a protein corona on the surface of extracellular vesicles in blood plasma[J]. J Extracell Vesicles, 2021, 10(11): e12140.
[5]
Dong L, Zieren RC, Horie K, et al. Comprehensive evaluation of methods for small extracellular vesicles separation from human plasma, urine and cell culture medium[J]. J Extracell Vesicles, 2020, 10(2): e12044.
[6]
Muraoka S, Jedrychowski MP, Yanamandra K, et al. Proteomic Profiling of Extracellular Vesicles Derived from Cerebrospinal Fluid of Alzheimer's Disease Patients: A Pilot Study[J]. Cells, 2020, 9(9): 1959.
[7]
Dixson AC, Dawson TR, Di Vizio D, et al. Context-specific regulation of extracellular vesicle biogenesis and cargo selection[J]. Nat Rev Mol Cell Biol, 2023, 24(7): 454-476.
[8]
Couch Y, Buzàs EI, Di Vizio D, et al. A brief history of nearly EV-erything - The rise and rise of extracellular vesicles[J]. J Extracell Vesicles, 2021, 10(14): e12144.
[9]
Zhang P, Dai M. CircRNA: a rising star in plant biology[J]. J Genet Genomics, 2022, 49(12): 1081-1092.
[10]
He YD, Tao W, He T, et al. A urine extracellular vesicle circRNA classifier for detection of high-grade prostate cancer in patients with prostate-specific antigen 2~10 ng/ml at initial biopsy[J]. Mol Cancer, 2021, 20(1): 96.
[11]
Garcia-Martin R, Wang G, Brandão BB, et al. MicroRNA sequence codes for small extracellular vesicle release and cellular retention[J]. Nature, 2022, 601(7893): 446-451.
[12]
Lazar S, Goldfinger LE. Platelets and extracellular vesicles and their cross talk with cancer[J]. Blood, 2021, 137(23): 3192-3200.
[13]
García-Silva S, Benito-Martín A, Nogués L, et al. Melanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism[J]. Nat Cancer, 2021, 2(12): 1387-1405.
[14]
Gagliardi D, Bresolin N, Comi GP, et al. Extracellular vesicles and amyotrophic lateral sclerosis: from misfolded protein vehicles to promising clinical biomarkers[J]. Cell Mol Life Sci, 2021, 78(2): 561-565.
[1] 王亚红, 蔡胜, 葛志通, 杨筱, 李建初. 颅骨骨膜窦的超声表现一例[J/OL]. 中华医学超声杂志(电子版), 2024, 21(11): 1089-1091.
[2] 唐金侨, 叶宇佳, 王港, 赵彬, 马艳宁. 医学影像学检查方法在颞下颌关节紊乱病中临床应用研究进展[J/OL]. 中华口腔医学研究杂志(电子版), 2024, 18(06): 406-411.
[3] 赵林娟, 吕婕, 王文胜, 马德茂, 侯涛. 超声引导下染色剂标记切缘的梭柱型和圆柱型保乳区段切除术的效果研究[J/OL]. 中华普外科手术学杂志(电子版), 2024, 18(06): 634-637.
[4] 熊鹰, 林敬莱, 白奇, 郭剑明, 王烁. 肾癌自动化病理诊断:AI离临床还有多远?[J/OL]. 中华腔镜泌尿外科杂志(电子版), 2024, 18(06): 535-540.
[5] 李伟, 宋子健, 赖衍成, 周睿, 吴涵, 邓龙昕, 陈锐. 人工智能应用于前列腺癌患者预后预测的研究现状及展望[J/OL]. 中华腔镜泌尿外科杂志(电子版), 2024, 18(06): 541-546.
[6] 祝炜安, 林华慧, 吴建杰, 黄炯煅, 吴婷婷, 赖文杰. RDM1通过CDK4促进前列腺癌细胞进展的研究[J/OL]. 中华腔镜泌尿外科杂志(电子版), 2024, 18(06): 618-625.
[7] 中华医学会器官移植学分会. 肝移植术后缺血性胆道病变诊断与治疗中国实践指南[J/OL]. 中华肝脏外科手术学电子杂志, 2024, 13(06): 739-748.
[8] 郑大雯, 王健东. 胆囊癌辅助诊断研究进展[J/OL]. 中华肝脏外科手术学电子杂志, 2024, 13(06): 769-773.
[9] 袁雨涵, 杨盛力. 体液和组织蛋白质组学分析在肝癌早期分子诊断中的研究进展[J/OL]. 中华肝脏外科手术学电子杂志, 2024, 13(06): 883-888.
[10] 董晓斌, 张静, 苏莎莎, 莎比亚·沙吾提, 盛好. 溃疡性结肠炎患者相关环状RNA 差异表达谱分析及功能研究[J/OL]. 中华结直肠疾病电子杂志, 2024, 13(06): 499-509.
[11] 李浩, 陈棋帅, 费发珠, 张宁伟, 李元东, 王硕晨, 任宾. 慢性肝病肝纤维化无创诊断的研究进展[J/OL]. 中华临床医师杂志(电子版), 2024, 18(09): 863-867.
[12] 谭瑞义. 小细胞骨肉瘤诊断及治疗研究现状与进展[J/OL]. 中华临床医师杂志(电子版), 2024, 18(08): 781-784.
[13] 王子阳, 王宏宾, 刘晓旌. 血清标志物对甲胎蛋白阴性肝细胞癌诊断的研究进展[J/OL]. 中华临床医师杂志(电子版), 2024, 18(07): 677-681.
[14] 陈慧, 邹祖鹏, 周田田, 张艺丹, 张海萍. 皮肤镜对头皮红斑性皮肤病辅助鉴别诊断的研究进展[J/OL]. 中华临床医师杂志(电子版), 2024, 18(07): 692-698.
[15] 胡云鹤, 周玉焯, 付瑞瑛, 于凡, 李爱东. CHS-DRG付费制度下GB1分组住院费用影响因素分析与管理策略探讨[J/OL]. 中华临床医师杂志(电子版), 2024, 18(06): 568-574.
阅读次数
全文


摘要


AI


AI小编
你好!我是《中华医学电子期刊资源库》AI小编,有什么可以帮您的吗?