The expression of growth associated protein 43 in bladder tissue of patients with interstitial cystitis

doi:10.3877/cma.j.issn.1674-3253.2018.02.005

Abstract

Abstract:

Objective

To explore the growth associated protein (GAP-43) expression in patients with interstitial cystitis/bladder painful syndrome (IC/BPS) and the correlation between symptoms.

Methods

Through the immune histochemical method to detect GAP-43 expression in bladder tissue of 31 patients with IC/BPS, ICSI, ICPI and PUF score before treatment, and the ralationship between GAP-43 expression and patient's symptoms were assessed.

Results

Of 31 IC/BPS patients, ICSI scores were ranged from 13 to 19 (mean 16±3), ICPI scores were ranged from 7 to 16 (mean 12±1), PUF scores were ranged from 16 to 25 (mean 20±2), symptom scores were ranged from 37 to 57 (46±4). The expression of GAP-43 immune histochemical scores were ranged from 1 to 6 (mean 5.2 ± 0.3) points. There was a positive correlation between GAP-43 expression levels in the bladder tissue and symptom severity of IC/BPS patients, correlation coefficients were 0.469, 0.611, 0.426 respectively, P value were 0.046, 0.039, 0.008 respectively.

Conclusion

GAP-43 expression in bladder tissue of IC/BPS patients are positively related to the patient's symptom severity, suggesting that GAP-43 expression in bladder tissue may be involved in the pathogenesis of IC/BPS.

Key words: Interstitial cystitis, GAP-43, Symptoms

Fei Yang, Jiarong Cai, Tengcheng Li, Bolong Liu, Hailun Zhan, Xiangfu Zhou. The expression of growth associated protein 43 in bladder tissue of patients with interstitial cystitis[J]. Chinese Journal of Endourology(Electronic Edition), 2018, 12(02): 86-89.

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References 17

[1]	Benowitz LI, Routtenberg A. GAP-43: an intrinsic determinant of neuronal development and plasticity[J]. Trends Neurosci, 1997, 20(2):84-91.
[2]	刘柏隆, 周祥福, 杨飞, 等. 间质性膀胱炎各层组织中神经纤维过表达及其临床意义[J/CD]. 中华腔镜泌尿外科杂志(电子版), 2013, 7(1): 50-54.
[3]	Gillenwater JY,Wein AJ.Summary of the National Institute of Arthritis, Diabetes, Digestive and Kidney Diseases Workshop on Interstitial Cystitis, National Institutes of Health, Bethesda, Maryland, August 28-29, 1987[J]. J Urol, 1988, 140(1): 203-206.
[4]	O'Leary MP, Sant GR, Fowler FJ Jr, et al. The interstitial cystitis symptom index and problem index[J]. Urology, 1997, 49 (5A Suppl): 58-63.
[5]	Parsons CL, Dell J, Stanford EJ, et al. Increased prevalence of interstitial cystitis: previously unrecognized urologic and gynecologic cases identified using a new symptom questionnaire and intravesical potassium sensitivity[J]. Urology, 2002, 60(4): 573-578.
[6]	Theoharides TC, Sant GR, el-Mansoury M, et al. Activation of bladder mast cells in interstitial cystitis: a light and electron microscopic study[J]. J Urol, 1995, 153(3 Pt 1): 629-636.
[7]	Christmas TJ, Rode J, Chapple CR, et al. Nerve fibre proliferation in interstitial cystitis[J]. Virchows Arch A PatholAnatHistopathol, 1990, 416(5):447-451.
[8]	Loy DN, Crawford CH, Darnall JB, et al. Temporal progression of angiogenesis and basal lamina deposition after contusive spinal cord injury in the adult rat[J]. J Comp Neurol, 2002, 445(4): 308-324.
[9]	Wakabayashi Y, Maeda T, Aimi Y, et al. Increase of low-affinity neurotrophin receptor p75 and growth-associated protein-43 immunoreactivities in the rat urinary bladder during experimentally induced nerve regeneration[J]. J Urol, 1998, 160(4): 1513-1517.
[10]	Schicho R, Schuligoi R, Sirinathsinghji DJ, et al. Increased expression of GAP-43 in small sensory neurons after stimulation by NGF indicative of neuroregeneration in capsaicin-treated rats[J]. RegulPept, 1999, 83(2-3): 87-95.
[11]	Vizzard MA, Boyle MM. Increased expression of growth-associated protein (GAP-43) in lower urinary tract pathways following cyclophosphamide (CYP)-induced cystitis[J]. Brain Res, 1999, 844(1-2): 174-187.
[12]	Lowe EM, Anand P, Terenghi G, et al. Increased nerve growth factor levels in the urinary bladder of women with idiopathic sensory urgency and interstitial cystitis[J]. Br J Urol, 1997, 79(4): 572-577.
[13]	Dupont MC, Spitsbergen JM, Kim KB, et al. Histological and neurotrophic changes triggered by varying models of bladder inflammation[J]. J Urol, 2001, 166(3): 1111-1118.
[14]	Chuang YC, Fraser MO, Yu Y, et al. The role of bladder afferent pathways in bladder hyperactivity induced by the intravesical administration of nerve growth factor[J]. J Urol, 2001, 165(3): 975-979.
[15]	Huang F, Liu Z, Liu H, et al. GM1 and NGF modulate Ca²⁺ homeostasis and GAP43 mRNA expression in cultured dorsal root ganglion neurons with excitotoxicity induced by glutamate[J]. NutrNeurosci, 2007, 10(3-4): 105-111.
[16]	梅红兵, 杜光辉, 叶章群，等. 生长相关蛋白-43在膀胱过度活动症患者膀胱表达的研究[J]. 中国医师杂志, 2006, 8(6): 724-726.
[17]	Donovan SL, Mamounas LA, Andrews AM, et al. GAP-43 is critical for normal development of the serotonergic innervation in forebrain[J]. J Neurosci, 2002, 22(9): 3543-3552.

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