Effect of dimethyl phthalate on kidney and reproductive system in rats

doi:10.3877/cma.j.issn.1674-3253.2019.02.015

Abstract

Abstract:

Objective

To study the effect of dimethyl phthalate on kidney and reproductive system in rats.

Methods

Sixty Wistar clean grade male rats were randomly divided into low dose group, middle dose group, high dose group and control group with 15 rats in each group. Serum creatinine (Cr), urea nitrogen bun (BUN), luteinizing hormone (LH), follicle stimulating hormone (FSH), testosterone (T), cystatin C (cys-C), β-2 microglobulin (β-Mg) were detected in the drug groups after 6 weeks of treatment.The changes of renal histomorphology were observed by HE staining. Single factor analysis of variance was used to compare the two groups.

Results

Serum creatinine and BUN increased significantly in high dose group (F=11.70, 4.16; P<0.05), while Cystatin C (Cys-C) and β-2-microglobulin (β-Mg) increased significantly in low, medium and high dose groups. (F=31.16, 8.05; P<0.05). Serum testosterone level decreased in high dose group, LH level increased in low dose group, but decreased in high dose group and middle dose group, FSH level increased in low, medium and high dose group (F=4.82, 59.34, 6.91; P<0.05). There was no obvious change in renal tissue structure in the control group and low dose group, while the glomerular atrophy occurred in the kidney in the middle dose group, and the glomerular atrophy, degeneration and necrosis occurred in the high dose group.

Conclusion

Dimethyl phthalate can induce the changes of reproductive endocrine hormone level and glomerular lesion in rats.

Key words: Dimethyl phthalate, Urinary system, Toxicity, Rat

Jie Wu, Xiqing Wu. Effect of dimethyl phthalate on kidney and reproductive system in rats[J]. Chinese Journal of Endourology(Electronic Edition), 2019, 13(02): 132-134.

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Figures/Tables 2

References 12

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组别	脏器系数		肾功能				生殖激素
组别	肾脏(×10^-3)	睾丸(×10^-3)	BUN(mmol/L)	Cr(μmol /L)	Cys-C(mg/L)	β-Mg(mg/L)	T(μg/L)	LH(IU/L)	FSH(IU/L)
对照组	18.6±1.9	7.7±1.5	5.3±1.5	077±8	0.77±0.11	1.9±0.4	3.02±1.31	6.36±0.92	1.68±0.65
低剂量组	17.5±1.6	7.0±2.0	5.9±1.9	085±10	0.97±0.09^a	2.29±0.7^a	2.68±1.40	8.24±1.85^a	2.65±0.52^a
中剂量组	17.4±1.6	6.1±1.8	6.1±1.9	097±10	1.13±0.15^a	2.6±0.7^a	2.42±1.19	3.16±1.24^a	2.29±0.89^a
高剂量组	19.7±1.4	6.8±1.1	7.6±2.1	105±23^a	1.42±0.32^a	3.0±0.7 ^a	1.45±0.76^a	2.52±1.25^a	2.75±0.74^a
F值	6.55	2.43	4.16	11.70	31.16	8.05	4.82	59.34	6.91
P值	<0.05	<0.05	<0.05	<0.05	<0.05	<0.05	<0.05	<0.05	<0.05

组别	脏器系数		肾功能				生殖激素
组别	肾脏(×10^-3)	睾丸(×10^-3)	BUN(mmol/L)	Cr(μmol /L)	Cys-C(mg/L)	β-Mg(mg/L)	T(μg/L)	LH(IU/L)	FSH(IU/L)
对照组	18.6±1.9	7.7±1.5	5.3±1.5	077±8	0.77±0.11	1.9±0.4	3.02±1.31	6.36±0.92	1.68±0.65
低剂量组	17.5±1.6	7.0±2.0	5.9±1.9	085±10	0.97±0.09^a	2.29±0.7^a	2.68±1.40	8.24±1.85^a	2.65±0.52^a
中剂量组	17.4±1.6	6.1±1.8	6.1±1.9	097±10	1.13±0.15^a	2.6±0.7^a	2.42±1.19	3.16±1.24^a	2.29±0.89^a
高剂量组	19.7±1.4	6.8±1.1	7.6±2.1	105±23^a	1.42±0.32^a	3.0±0.7 ^a	1.45±0.76^a	2.52±1.25^a	2.75±0.74^a
F值	6.55	2.43	4.16	11.70	31.16	8.05	4.82	59.34	6.91
P值	<0.05	<0.05	<0.05	<0.05	<0.05	<0.05	<0.05	<0.05	<0.05

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