研究长链脂酰辅酶A合成酶3（ACSL3）表达对前列腺癌（PCa）细胞增殖能力的影响，探索ACSL3调控PI3K/Akt/MMP-9信号通路的分子机制，发掘ACSL3预测前列腺癌复发进展的临床应用价值。

利用Western blot检测ACSL3在不同前列腺癌细胞系中的表达；构建稳定表达ACSL3的PCa细胞株，利用MTT法检测过表达ACSL3对PCa细胞增殖的改变；Western blot检测过表达ACSL3对PCa细胞中Akt，磷酸化Akt（p-Akt），基质金属蛋白酶9（MMP-9）表达水平的影响；通过免疫荧光染色实验探索ACSL3与Akt蛋白之间是否可能存在共定位；利用免疫组织化学法（IHC）比较不同Gleason评分患者中ACSL3的表达差异。

Western blot检测显示ACSL3蛋白在局限性前列腺癌细胞22Rv1中存在着特异性的低表达，同时，ACSL3蛋白在激素非依赖性前列腺癌细胞中较激素依赖性前列腺癌细胞表达量更高。MTT实验表明过表达ACSL3后癌细胞增殖能力明显增强。此外，Western blot显示过表达ACSL3后p-Akt、MMP-9表达均明显上调；激光共聚焦显微镜下，免疫荧光染色显示ACSL3与Akt存在着蛋白共定位关系。临床检测中，IHC显示ACSL3表达量随Gleason评分升高而增加。

ACSL3可能通过与Akt蛋白间的相互作用，介导PI3K/Akt/MMP-9信号通路的激活，影响PCa细胞增殖。此外，ACSL3的表达与前列腺癌的Gleason评分具有相关性，可能影响患者预后。

To investigate the molecular mechanism of ACSL3 expression in enhancing prostate cancer (PCa) cell proliferation, and to explore the potential value of ACSL3 expression in prognosis of the recurrence and metastasis of PCa.

Western blot was used to detect the expression of ACSL3 in different PCa cell lines. The pCDNA3.1(+)-Flag-ACSL3 plasmids were transfected into prostate cancer cells. And then, MTT assay was used to detect the alteration of cell proliferation. After over expression of ACSL3, western blot was used to detect the expression of Akt, phosphorylated Akt (p-Akt) and Matrix Metalloproteinase-9 (MMP-9) in cancer cells. Immunofluorescence staining was carried out to detect whether there was a co-localization between ACSL3 and Akt protein. Immunohistochemistry (IHC) assay was used to evaluate ACSL3 expression in tissues of PCa patients with different Gleason score.

Western blot showed that ACSL3 protein got lower expression in localized prostate cancer cell line 22Rv1, while higher expression in hormone-independent PCa cells than in hormone-dependent PCa cells. The MTT assay demonstrated that the cell proliferation was significantly increased after ACSL3 overexpression. Moreover, overexpressing ACSL3 can also enhance p-Akt, MMP-9 expression. Immunofluorescence staining implied that there was a co-localization between ACSL3 and Akt in 22Rv1. In addition, IHC results revealed that the expression of ACSL3 protein was enhanced with the increasing of Gleason score, result in higher ACSL3 expression.

ACSL3 may induce the activation of PI3K/Akt/MMP-9 signaling pathway via the interaction between ACLS3 and Akt protein, which can enhance the proliferation of prostate cancer cells. Meanwhile, the increase expression of ACSL3 protein was correlated with higher Gleason score in patients with PCa.