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中华腔镜泌尿外科杂志(电子版) ›› 2020, Vol. 14 ›› Issue (06) : 460 -465. doi: 10.3877/cma.j.issn.1674-3253.2020.06.014

所属专题： 文献；

实验研究

PFKFB3基因在前列腺癌中的表达及其对前列腺癌细胞糖酵解及生长的影响
李骏1, 张倩2, 江东根1, 陈楚杰1, 庞俊1,†()   
  1. 1. 518107　深圳，中山大学附属第七医院泌尿外科
    2. 518107　深圳，中山大学附属第七医院康复医学科
  • 收稿日期:2020-03-25 出版日期:2020-12-01
  • 通信作者: 庞俊
  • 基金资助:
    国家自然科学基金资助项目(81772754、81902613); 广东省自然科学基金重大基础研究培育项目(2017A03038009); 广东省医学科学技术研究基金资助项目(A2019555、A2019490)

Expression of PFKFB3 in prostate cancer and its effect on glycolysis and growth of prostate cancer cells

Jun Li1, Qian Zhang2, Donggen Jiang1, Chujie Chen1, Jun Pang1,†()   

  1. 1. Department of Urology, the Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen 508107, China
    2. Department of Rehabilitation Medicine, the Seventh Affiliated Hospital, Sun Yat-sen University, Shenzhen 508107, China
  • Received:2020-03-25 Published:2020-12-01
  • Corresponding author: Jun Pang
  • About author:
    Corresponding author: Pang Jun, Email:
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引用本文：

李骏, 张倩, 江东根, 陈楚杰, 庞俊. PFKFB3基因在前列腺癌中的表达及其对前列腺癌细胞糖酵解及生长的影响[J/OL]. 中华腔镜泌尿外科杂志(电子版), 2020, 14(06): 460-465.

Jun Li, Qian Zhang, Donggen Jiang, Chujie Chen, Jun Pang. Expression of PFKFB3 in prostate cancer and its effect on glycolysis and growth of prostate cancer cells[J/OL]. Chinese Journal of Endourology(Electronic Edition), 2020, 14(06): 460-465.

目的

探讨6-磷酸果糖激酶-2/果糖双磷酸酶-2同工酶3（PFKFB3）基因在前列腺癌中的表达及其对前列腺癌细胞糖酵解及生长的影响。

方法

收集我院病理科前列腺增生和前列腺癌蜡块组织，应用免疫组化技术检测PFKFB3的表达水平。通过荧光定量PCR和Western blot实验检测正常前列腺上皮细胞（RWPE-1）和四种前列腺癌细胞系（PC3、LNCaP、DU145、C4-2）中PFKFB3的表达。应用RNA干扰技术敲低PFKFB3表达，采用细胞糖酵解试剂盒、CCK-8和克隆形成实验检测PFKFB3对前列腺癌细胞的糖酵解和增殖活性的影响。

结果

与前列腺增生组织相比，前列腺癌组织中的PFKFB3表达量明显增高[（59.7±0.25） vs （3.08±0.16），P<0.05]，且病理Gleason评分越高，PFKFB3表达量也越高。同样PFKFB3在不同前列腺癌细胞系中均明显高表达。抑制PFKFB3基因表达后，前列腺癌细胞的糖酵解和增殖能力显著降低。

结论

PFKFB3基因在前列腺癌恶性进展中表达上调，促进肿瘤细胞的糖酵解和增殖，靶向PFKFB3可能为前列腺癌分子诊断和治疗提供潜在的应用价值。

关键词: 前列腺癌, 细胞系, 6-磷酸果糖激酶-2/果糖双磷酸酶-2同工酶3, RNA干扰, 糖酵解, 增殖
Objective

To investigate the expression of 6-phosphofructo-2-kinase /fructose-2,6-biphosphatase 3 (PFKFB3) in prostate cancer and its effect on glycolysis and growth of prostate cancer cells.

Methods

Paraffin embedded tissues of benign prostatic hyperplasia (BPH) and prostate cancer in the Department of Pathology in our hospital were collected, and the expression level of PFKFB3 was detected by immunohistochemistry. PFKFB3 expression in benign prostatic epithelial cells (RWPE-1) and four prostate cancer cell lines (PC3, LNCaP, DU145, C4-2) was detected by fluorescence quantitative PCR and Western blotting. RNA interference was used to knock down the expression of PFKFB3, and the effects of PFKFB3 on the glycolysis and proliferation of prostate cancer cells were detected by cell glycolysis kit, CCK-8 and clonal formation assay.

Results

The expression level of PFKFB3 in prostate cancer tissues is significantly higher than that in benign prostatic hyperplasia tissues [(5.97±0.25) vs (3.08±0.16), P<0.05], and the expression level of PFKFB3 increases with the higher pathological Gleason score. PFKFB3 is also highly expressed in different prostate cancer cell lines. Inhibition of PFKFB3 significantly reduces glycolysis and proliferation of prostate cancer cells.

Conclusion

PFKFB3 gene is up-regulated in the malignant progression of prostate cancer and promotes glycolysis and proliferation of tumor cells. Targeting PFKFB3 may provide potential application value for molecular diagnosis and treatment of prostate cancer.

Key words: Prostate cancer, Cell line, 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3), RNA interference, Glycolysis, Proliferation
图1 PFKFB3在前列腺增生和前列腺癌组织中的表达
图2 PFKFB3在前列腺癌细胞系中的表达
图3 转染PFKFB3-shRNA后PFKFB3的表达
图4 PFKFB3-shRNA抑制前列腺癌细胞的糖酵解和增殖
[1]
Siegel RL, Miller KD, Jemal A. Cancer statistics, 2019[J]. CA Cancer J Clin, 2019，69(1): 7-34.
[2]
陈东, 李志勇, 李永红, 等. 机器人辅助前列腺癌根治术对比开放前列腺癌根治术:孰优孰劣?[J/CD]. 中华腔镜泌尿外科杂志（电子版）, 2019, 13(4): 217-220.
[3]
郑琛琛, 周祥福. 2019版欧洲泌尿外科前列腺癌指南更新要点解读[J/CD]. 中华腔镜泌尿外科杂志（电子版）, 2019, 13(6): 361-364.
[4]
Koppenol WH, Bounds PL, Dang CV. Otto Warburg's contributions to current concepts of cancer metabolism[J]. Nat Rev Cancer, 2011, 11(5): 325-337.
[5]
Vander Heiden MG, Cantley LC, Thompson CB. Understanding the Warburg effect: the metabolic requirements of cell proliferation[J]. Science, 2009, 324(5930): 1029-1033.
[6]
黄应龙, 左毅刚, 王剑松, 等.肿瘤代谢重编程及其在膀胱癌研究中的进展[J]. 中华实验外科杂志,2019, 36(12): 2312-2316.
[7]
Hay N. Reprogramming glucose metabolism in cancer: can it be exploited for cancer therapy? [J]. Nat Rev Cancer, 2016, 16(10): 635-649. Review.
[8]
Potter M, Newport E, Morten KJ. The Warburg effect: 80 years on[J]. Biochem Soc Trans, 2016, 44(5): 1499-1505.
[9]
Gatenby RA, Gillies RJ. Why do cancers have high aerobic glycolysis? [J]. Nat Rev Cancer, 2004, 4(11): 891-899.
[10]
Yi W, Clark PM, Mason DE, et al. Phosphofructokinase 1 glycosylation regulates cell growth and metabolism[J]. Science, 2012, 337(6097): 975-980.
[11]
Ros S, Schulze A. Balancing glycolytic flux: the role of 6-phosphofructo-2-kinase /fructose 2,6-bisphosphatases in cancer metabolism[J]. Cancer Metab, 2013, 1(1): 1-8.
[12]
Yalcin A, Telang S, Clem B, et al. Regulation of glucose metabolism by 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatases in cancer[J]. Exp Mol Pathol, 2009, 86 (3): 174-179.
[13]
O'Neal J, Clem A, Reynolds L, et al. Inhibition of 6-phosphofructo-2-kinase (PFKFB3) suppresses glucose metabolism and the growth of HER2+ breast cancer[J]. Breast Cancer Res Treat, 2016, 160(1): 29-40.
[14]
Shi L, Pan H, Liu Z, et al. Roles of PFKFB3 in cancer[J]. Signal Transduct Target Ther, 2017, 2: 17044.
[15]
Xing Z, Zhang Y, Liang K, et al. Expression of long noncoding rna yiya promotes glycolysis in breast cancer[J]. Cancer Res, 2018, 78(16): 4524-4532.
[16]
Han J, Meng Q, Xi Q, et al. PFKFB3 was overexpressed in gastric cancer patients and promoted the proliferation and migration of gastric cancer cells[J]. Cancer Biomark, 2017, 18(3): 249-256.
[17]
Zhou L, Zhan ML, Tang Y, et al. Effects of β-caryophyllene on arginine ADP-ribosyltransferase 1-mediated regulation of glycolysis in colorectal cancer under high-glucose conditions[J]. Int J Oncol, 2018, 53(4): 1613-1624.
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