Expression of GTSE1 in prostate cancer tissues and its regulation effect on the metastasis of prostate cancer cells

doi:10.3877/cma.j.issn.1674-3253.2022.03.017

Abstract

Abstract:

Objective

To investigate the expression of G2 and S phase-expressed-1 (GTSE1) in prostate cancer(PCa) tissues and its regulation in PCa metastasis.

Methods

GEPIA, UALCAN and FireBrowse databases were analyzed to determine the correlation between GTSE1 expression and clinical indexes and metastasis in patients with PCa. The co-expression genes and possible pathways of GTSE1 were further analyzed. Wound healing and transwell invasion assays were used to detect the effect of GTSE1 on the migration and invasion of PCa cells in vitro. Western blotting assay was used to analyze the effects of GTSE1 on epithelial-to-mesenchymal transition (EMT) markers.

Results

The expression of GTSE1 was significantly increased in PCa tissues, especially in metastatic PCa, and was closely related to the disease progression and lymph node metastasis. Overexpression of GTSE1 enhanced the ability of migration and invasion of PCa cells, and promoted the formation of EMT, which showed the opposite effects with GTSE1 knockdown. GTSE1 may act on the microtubules of PCa cells and cooperate with TPX2, TOP2A and CENPF to drive EMT and metastasis of PCa.

Conclusion

GTSE1 is highly expressed in PCa tissues and can promote the invasion and migration of PCa cells, which may be developed as a new molecular target for the treatment of metastatic PCa.

Key words: PCa, GTSE1, EMT, Metastasis

Wei'an Zhu, Wenjie Lai, Xiaojuan Li, Jiayu Zheng, Kun Bao, Youdi Cai, Limei Zhang, Jiongduan Huang, Xingqiao Wen. Expression of GTSE1 in prostate cancer tissues and its regulation effect on the metastasis of prostate cancer cells[J]. Chinese Journal of Endourology(Electronic Edition), 2022, 16(03): 270-275.

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References 23

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