Home    中文  
 
  • Search
  • lucene Search
  • Citation
  • Fig/Tab
  • Adv Search
Just Accepted  |  Current Issue  |  Archive  |  Featured Articles  |  Most Read  |  Most Download  |  Most Cited

Chinese Journal of Endourology(Electronic Edition) ›› 2024, Vol. 18 ›› Issue (06): 626-632. doi: 10.3877/cma.j.issn.1674-3253.2024.06.015

• Experimental Research • Previous Articles     Next Articles

Study on the killing effect of oncolytic virus M1 on different prostate cancer cells

Gongwei Wang1, Shuhao Li1, Song Wei1, Boran Lyu1, Cheng Hu1,()   

  1. 1.Department of Urology,the Third Affiliated Hospital of Sun Yat-sen University,Guangzhou 510630,China
  • Received:2024-09-05 Online:2024-12-01 Published:2024-11-26
  • Contact: Cheng Hu

Abstract:

Objective

To explore the differences and specific mechanisms of the killing effect of oncolytic virus M1 on different prostate cancer cells.

Methods

The survival rate of 13 different prostate cancer cells treated with M1 virus at different titers (0,0.001,0.01,0.1,1,10 PFU/CELL) for 48 hours was detected by MTT assay. Detection of gene expression in 13 different prostate cancer cells through transcriptome sequencing. Spearman/Person correlation analysis on the correlation between cell survival rate and gene expression level. Verify the correlation analysis results through protein labeling (Western Blot) and gene silencing (siRNA).

Results

The MTT assay showed that there were differences in the survival rates of 13 different prostate cancer cells under the same titer of M1 virus. Correlation analysis revealed that the MAPK14 gene was a key factor affecting the differential killing effect of M1 virus on different prostate cancer cells. Western blot showed that compared with the control group,the expression level of MAPK14 in siRNA group cells was significantly downregulated. The MTT assay showed that compared with the control group,the survival rate of cells in the siRNA group was significantly reduced under M1 virus infection (P<0.05).

Conclusion

MAPK14 is a key factor affecting the killing effect of M1 virus on prostate cancer cells. Targeted inhibition of MAPK14 can enhance the killing effect of M1 virus on prostate cancer cells.

Key words: Oncolytic virus, Prostate cancer, MAPK14

京ICP 备07035254号-20
Copyright © Chinese Journal of Endourology(Electronic Edition), All Rights Reserved.
Tel: 020-85252990 E-mail: chinendourology@126.com
Powered by Beijing Magtech Co. Ltd