NRCAM-PI3K-AKT pathway is involved in the miR-505 induced proliferative suppression of prostate cancer cells

doi:10.3877/cma.j.issn.1674-3253.2020.02.015

Abstract

Abstract:

Objective

Our previous study showed that miR-505 can suppress the proliferation, invasion and migration of prostate cancer (PCa) cells by targeting NRCAM. This study is aimed to investigate the role of miR-505 in PCa xenograft mouse models and its downstream signaling that miR-505/NRCAM may be mediated.

Methods

The xenograft mouse models of PCa was used to detect effects of miR-505 on the proliferative ability and the possible downstream pathway. After depletion of NRCAM in PC3 and LNCaP cells, CCK8 assays and Western blotting were chosen to identify the cell proliferation and the activities of PI3K/AKT pathways.

Results

Compared to the control group, the tumor volume and growth rate were significantly slower in PCa xenografts with miR-505 overexpression. Immunohistochemical analysis showed that the PCa xenografts overexpressing miR-505 have significantly lower AKT and EGFR protein than the control xenografts. We further showed that the cells with si-NrCAM transfection exhibited lower viability when compared with the control cell.

Conclusion

Our study may unveil the inhibitory effects of miR-505 on PCa proliferation, possibly by targeting NRCAM via PI3K/AKT signaling.

Key words: Prostate cancer, NRCAM, miR-505, PI3K-AKT pathway, Proliferation

Xiaohui Ling, Jiahong Chen, Zhiyun Chen, Shanwen Zhu, Shengbang Yang, Funeng Jiang, Weide Zhong. NRCAM-PI3K-AKT pathway is involved in the miR-505 induced proliferative suppression of prostate cancer cells[J]. Chinese Journal of Endourology(Electronic Edition), 2020, 14(02): 145-148.

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https://zhqjmnmwkzz.cma-cmc.com.cn/EN/Y2020/V14/I02/145

Figures/Tables 2

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