Expression of PFKFB3 in prostate cancer and its effect on glycolysis and growth of prostate cancer cells

doi:10.3877/cma.j.issn.1674-3253.2020.06.014

Abstract

Abstract:

Objective

To investigate the expression of 6-phosphofructo-2-kinase /fructose-2,6-biphosphatase 3 (PFKFB3) in prostate cancer and its effect on glycolysis and growth of prostate cancer cells.

Methods

Paraffin embedded tissues of benign prostatic hyperplasia (BPH) and prostate cancer in the Department of Pathology in our hospital were collected, and the expression level of PFKFB3 was detected by immunohistochemistry. PFKFB3 expression in benign prostatic epithelial cells (RWPE-1) and four prostate cancer cell lines (PC3, LNCaP, DU145, C4-2) was detected by fluorescence quantitative PCR and Western blotting. RNA interference was used to knock down the expression of PFKFB3, and the effects of PFKFB3 on the glycolysis and proliferation of prostate cancer cells were detected by cell glycolysis kit, CCK-8 and clonal formation assay.

Results

The expression level of PFKFB3 in prostate cancer tissues is significantly higher than that in benign prostatic hyperplasia tissues [(5.97±0.25) vs (3.08±0.16), P<0.05], and the expression level of PFKFB3 increases with the higher pathological Gleason score. PFKFB3 is also highly expressed in different prostate cancer cell lines. Inhibition of PFKFB3 significantly reduces glycolysis and proliferation of prostate cancer cells.

Conclusion

PFKFB3 gene is up-regulated in the malignant progression of prostate cancer and promotes glycolysis and proliferation of tumor cells. Targeting PFKFB3 may provide potential application value for molecular diagnosis and treatment of prostate cancer.

Key words: Prostate cancer, Cell line, 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3), RNA interference, Glycolysis, Proliferation

Jun Li, Qian Zhang, Donggen Jiang, Chujie Chen, Jun Pang. Expression of PFKFB3 in prostate cancer and its effect on glycolysis and growth of prostate cancer cells[J]. Chinese Journal of Endourology(Electronic Edition), 2020, 14(06): 460-465.

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References 17

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