Profiling study of NURR1-overexpressed LNCaP cells by gene chip analysis

doi:10.3877/cma.j.issn.1674-3253.2022.01.018

Abstract

Abstract:

Objective

To investigate the profiling of NURR1-overexpressed LNCaP cells by gene chip analysis.

Methods

NURR1 was ectopic expressed in LNCaP cells by lentivirus infection, validated by immunocytochemical staining. The profiling of mRNA and lncRNA was compared by gene chips between NURR1-overexpressed LNCaP cells and vector-transfected control. KEEG and GO analysis were performed to study the potential functional role and signaling pathways involved.

Results

A total of 515 genes were differentially expressed in NURR1-overexpressed LNCaP cells as compared to its vector-transfected control, including 238 mRNA upregulated, 195 mRNA down regulated, 54 lncRNA upregulated and 28 lncRNA downregulated. The differentially expressed mRNA and lncRNA were mainly involved in molecular functions, among which the retinal binding function, iron ion binding capacity, nerve axon regulation and redox process, and the cytological composition of the extracellular region exhibited the most significance. The interaction between cytokines and cytokine receptors, as well as stem cell pluripotency related signaling pathways were changed significantly.

Conclusion

The mRNA and lncRNA profiling has been significantly changed by the ectopic expression of NURR1, which might play critical role in the development progression of prostate cancer.

Key words: Orphan nuclear receptor, NURR1, Prostate cancer, Gene expression profile

Rui Sun, Hui Liang, Ying Zhang, Qiong Deng, Qiyi Hu, Shengping Zhang, Jianwen Zhang, Zhu Wang. Profiling study of NURR1-overexpressed LNCaP cells by gene chip analysis[J]. Chinese Journal of Endourology(Electronic Edition), 2022, 16(01): 81-85.

/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://zhqjmnmwkzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.1674-3253.2022.01.018

https://zhqjmnmwkzz.cma-cmc.com.cn/EN/Y2022/V16/I01/81

Figures/Tables 6

References 16

[1]	姜涛. 孤儿核受体在前列腺癌中的研究进展[J]. 实用肿瘤学杂志, 2018, 32(4): 377-380.
[2]	Yao H, Yang L, Tian L, et al. LncRNA MSC-AS1 aggravates nasopharyngeal carcinoma progression by targeting miR-524-5p/nuclear receptor subfamily 4 group A member 2 (NR4A2)[J]. Cancer Cell Int, 2020, 20:138.
[3]	Inamoto T, Czerniak BA, Dinney CP, et al. Cytoplasmic mislocalization of the orphan nuclear receptor Nurr1 is a prognostic factor in bladder cancer[J]. Cancer, 2010, 116(2): 340-346.
[4]	Llopis S, Singleton B, Duplessis T, et al. Dichotomous roles for the orphan nuclear receptor NURR1 in breast cancer[J]. BMC Cancer, 2013, 13: 139.
[5]	Karki K, Li X, Jin UH, et al. Nuclear receptor 4A2 (NR4A2) is a druggable target for glioblastomas[J]. J Neurooncol, 2020, 146(1): 25-39.
[6]	Guo J, Zu G, Zhou T, et al. Clinicopathological significance of orphan nuclear receptor Nurr1 expression in gastric cancer[J]. Clin Transl Oncol, 2015, 17(10): 788-794.
[7]	Wang J, Yang J, Zou Y, et al. Orphan nuclear receptor nurr1 as a potential novel marker for progression in human prostate cancer[J]. Asian Pac J Cancer Prev, 2013, 14(3): 2023-2028.
[8]	胡七一, 张建文, 王宏亮, 等. 孤儿核受体NURR1在前列腺癌中的表达以及功能研究[J]. 现代泌尿外科杂志, 2018, 23(4): 299-303.
[9]	张建文, 王宏亮, 邓琼, 等. 核受体RORβ在前列腺癌细胞中的表达及其对肿瘤多细胞球形成的影响[J]. 现代泌尿外科杂志, 2019, 24(8): 667-672.
[10]	王铸, 张建文, 张颖, 等. 草酸钙结石晶体粘附对人肾上皮细胞lncRNA表达谱的影响[J]. 现代泌尿外科杂志, 2018, 23(1): 52-56, 76.
[11]	张建文, 徐长志, 张颖, 等. 草酸钙结石晶体对人近曲肾小管上皮细胞蛋白质表达谱的影响[J/CD]. 中华腔镜泌尿外科杂志（电子版）, 2019, 13(4): 225-229.
[12]	Wu Y, Liu ZG, Shi MQ, et al. Identification of ACTG2 functions as a promoter gene in hepatocellular carcinoma cells migration and tumor metastasis[J]. Biochem Biophys Res Commun, 2017, 491(2): 537-544.
[13]	Edfeldt K, Hellman P, Westin G, et al. A plausible role for actin gamma smooth muscle 2 (ACTG2) in small intestinal neuroendocrine tumorigenesis[J]. BMC Endocr Disord, 2016, 16: 19.
[14]	Schutyser E, Struyf S, Van Damme J. The CC chemokine CCL20 and its receptor CCR6[J]. Cytokine Growth Factor Rev, 2003, 14(5): 409-426.
[15]	Rubie C, Frick VO, Ghadjar P, et al. CCL20/CCR6 expression profile in pancreatic cancer[J]. J Transl Med, 2010, 8: 45.
[16]	Liu B, Jia Y, Ma J, et al. Tumor-associated macrophage-derived CCL20 enhances the growth and metastasis of pancreatic cancer[J]. Acta Biochim Biophys Sin (Shanghai), 2016, 48(12): 1067-1074.

类别	基因	登记编号	差异倍数	P值
mRNA	ACTG2	NM_001615	40.6	0.0003
	HTR5A	NM_024012	35	0.0007
	MYOD1	NM_002478	34.9	0.0014
	PLOD2	NM_182943	29.7	0
	OR51E1	NM_152430	20.7	0.0004
	CCL20	NM_004591	-11.8	0
	MTRNR2L2	NM_001190470	-11.3	0.0008
	MATN4	NM_003833	-10.6	0.0003
lncRNA	HTR5A-AS1	NR_038945	23	0
	LOC399715	NR_040079	17.7	0.0005
	LOC100129931	NR_033828	12.9	0.0002
	LINC01291	NR_125792	10.4	0.0021
	LOC100996455	NR_073177	10.3	0.0026
	LINC01023	NR_046368	10	0.0016
	LINC00240	NR_026775	-11.1	0.0138
	LOC440896	NR_015361	-9.2	0.0044
	LOC100131047	NR_038401	-8.1	0.0001

类别	基因	登记编号	差异倍数	P值
mRNA	ACTG2	NM_001615	40.6	0.0003
	HTR5A	NM_024012	35	0.0007
	MYOD1	NM_002478	34.9	0.0014
	PLOD2	NM_182943	29.7	0
	OR51E1	NM_152430	20.7	0.0004
	CCL20	NM_004591	-11.8	0
	MTRNR2L2	NM_001190470	-11.3	0.0008
	MATN4	NM_003833	-10.6	0.0003
lncRNA	HTR5A-AS1	NR_038945	23	0
	LOC399715	NR_040079	17.7	0.0005
	LOC100129931	NR_033828	12.9	0.0002
	LINC01291	NR_125792	10.4	0.0021
	LOC100996455	NR_073177	10.3	0.0026
	LINC01023	NR_046368	10	0.0016
	LINC00240	NR_026775	-11.1	0.0138
	LOC440896	NR_015361	-9.2	0.0044
	LOC100131047	NR_038401	-8.1	0.0001

Please choose a citation manager

Content to export